黄 盼^1，2，曹 菁^1，2*，王忠稳^1，2，郝江伟^1，2，王 晨^1，2

（1.劲牌持正堂药业有限公司，黄石435000；2.中药保健食品与安全湖北省重点实验室，大冶435100）

摘要：通过HPLC 测定参芪复合固体饮料中人参皂苷Rg1、甘草酸、黄芪甲苷的含量，并进行加速试验考察其稳定性。结果表明，人参皂苷Rg1、甘草酸、黄芪甲苷分别在3.170 9～63.418 6 μg/mL、0.794 5～15.899 3 μg/mL、20.319 9～203.199 3 μg/mL浓度范围内线性良好，加标回收率范围分别为100.27%～104.87%、100.74%～104.72%、103.07%～107.54%。在3个月加速试验中，该固体饮料性状、感官、溶解度均符合要求；3种成分含量均呈现出良好的稳定性。表明参芪复合固体饮料中人参皂苷Rg1、甘草酸、黄芪甲苷的含量在3个月加速试验中相对稳定，该研究可为参芪复合固体饮料的有效期制定提供良好依据。

关键词：固体饮料；人参皂Rg1；甘草酸；黄芪甲苷；稳定性；高效液相色谱

中图分类号：TS207.3 文献标识码：A 文章编号：1674-506X（2024）05-0127-0006

Study on the Establishment of Detection Methods and Content Stability of Three Effective Components in Shenqi Compound Solid Beverage

HUANG Pan^1，2，CAO Jing^1，2*，WANG Zhongwen^1，2，HAO Jiangwei^1，2，WANG Chen^1，2

（1.Jing Brand Chizhengtang Pharmaceutical Co.，Ltd.，Huangshi 435000，China；2.Hubei Provincial Key Laboratory for Quality and Safety of Traditional Chinese Medicine Health Food，Jing Brand Co.，Ltd.，Daye 435100，China）

Abstract：HPLC was used to determine the content of ginsenoside Rg1，glycyrrhizic acid，and astragaloside IV in Shenqi compound solid beverage， and their stability was investigated through accelerated experiment. The results showed that ginsenoside Rg1， glycyrrhizic acid， and astragaloside IV had a good linear relation in the respective concentration range of 3.1709～63.4186μg/mL，0.7945～15.8993μg/mL and 20.3199～203.1993μg/mL，the range of sample recovery rates were 100.27%～104.87% ， 100.74%～104.72%， 103.07%～107.54%，respectively. 3-month accelerated experiment showed that textural properties， sensory properties， and solubility of the solid beverage met the requirements； The content of all three components showed good stability. This indicates the stability of ginsenoside Rg1， glycyrrhizic acid， and astragaloside IV in Shenqi compound solid beverage was good in 3-month accelerated experiment，this study provides a good reference for determining the expiration date of Shenqi compound solid beverage.

Keywords：solid beverage；ginsenoside Rg1；glycyrrhizic acid；astragaloside IV；stabilities；HPLC

doi：10.3969/j.issn.1674-506X.2024.05-020

收稿日期：2023-10-09

基金项目：湖北省科技重大专项课题（2020ACA007）

作者简介：黄盼（1989-），女，硕士，工程师。研究方向：中药保健食品。

*通信作者：曹菁（1987-），女，硕士，工程师。研究方向：中药保健食品。